https://ogma.newcastle.edu.au/vital/access/ /manager/Index en-au 5 Small changes in environmental parameters lead to alterations in antibiotic resistance, cell morphology and membrane fatty acid composition in staphylococcus lugdunensis https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:16825 Wed 11 Apr 2018 14:14:42 AEST ]]> Development of a complex amino acid supplement, Fatigue Reviva (TM), for oral ingestion: initial evaluations of product concept and impact on symptoms of sub-health in a group of males https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:14316 Wed 11 Apr 2018 09:34:43 AEST ]]> Sweat facilitated amino acid losses in male athletes during exercise at 32-34 °C https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:27775 Thu 07 Feb 2019 14:52:24 AEDT ]]> Evaluation of in-situ fatty acid extraction protocols for the analysis of staphylococcal cell membrane associated fatty acids by gas chromatography https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:46546 Fri 25 Nov 2022 11:26:26 AEDT ]]> Environmentally driven changes in fatty acid profiles of a commercially important Penaeid prawn https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37144 Fri 21 Aug 2020 12:35:43 AEST ]]> Changes in the cytoplasmic composition of amino acids and proteins observed in Staphylococcus aureus during growth under variable growth conditions representative of the human wound site https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:27773 Staphylococcus aureus is an opportunistic pathogen responsible for a high proportion of nosocomial infections. This study was conducted to assess the bacterial responses in the cytoplasmic composition of amino acids and ribosomal proteins under various environmental conditions designed to mimic those on the human skin or within a wound site: pH6-8, temperature 35-37°C, and additional 0-5% NaCl. It was found that each set of environmental conditions elicited substantial adjustments in cytoplasmic levels of glutamic acid, aspartic acid, proline, alanine and glycine (P< 0.05). These alterations generated characteristic amino acid profiles assessed by principle component analysis (PCA). Substantial alterations in cytoplasmic amino acid and protein composition occurred during growth under conditions of higher salinity stress implemented via additional levels of NaCl in the growth medium. The cells responded to additional NaCl at pH 6 by reducing levels of ribosomal proteins, whereas at pH 8 there was an upregulation of ribosomal proteins compared with the reference control. The levels of two ribosomal proteins, L32 and S19, remained constant across all experimental conditions. The data supported the hypothesis that the bacterium was continually responding to the dynamic environment by modifying the proteome and optimising metabolic homeostasis.]]> Fri 05 Aug 2022 15:55:12 AEST ]]>